ScholarLens Back to search
2013•American Journal of BioScienceOpen access

Comparison of Diagnostic Methods in Cutaneous Leishmaniasis in Iraq

Abdulsadah Abdulabbas Rahi

Open full text 19 citations

Abstract

Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.

Open-access reader

About this research paper

What this paper is about

Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.

Why it matters

OpenAlex reports 19 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.

Key concepts: Giemsa stain, Cutaneous leishmaniasis, Amastigote, Leishmania tropica, Leishmaniasis, Leishmania, Leishmania major, Medicine

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Comparison of Diagnostic Methods in Cutaneous Leishmaniasis in Iraq — Research Paper | ScholarLens