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Some Observation on the Column Chromatography of Formosan Cobra Venom (Naja naja atra CANTOR)

Sadaaki Iwanaga, Chen-Chung Yang, Saju Kawachi

Abstract

Phosphodiesterase, phosphomonoesterase, 5′-nucleotidase, and lecithinase-A are markedly stable in cobra venom and can be preserved over a long period. Two kinds of phosphodiesterase are present in fresh cobra venom immediately after collection. Two kinds of strongly basic protein are also present in the cobra venom and the two kinds of substance related to nucleic acid, having absorption maxima at around 252 and 257mμ, present in the venom can be separated from each other by column chromatography using carboxymethylcellulose.

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Available abstract

Key concepts: Naja, Cobra, Venom, Chromatography, Chemistry, Phosphodiesterase, Column chromatography, Lecithinase