Isolation of Anthraniloylanthranilic Acid as the Metabolic Products of Anthranilic Acid

From the urine of a rabbit administered with 1g./kg. weight of anthranilic acid, unchanged acid and bimolar-bonded acid, anthraniloylanthranilic acid, were isolated and confirmed. This detoxication mechanism is a kind of peptide bonding but a peptide bonding of two moles of anthranilic acid is a new observation. Formation of anthraniloylanthranilic acid in rabbits is very marked but the amount formed is very small in rats, indicating a difference between animals. Apart from the above, 5-hydroxyanthranilic acid, o-aminohippuric acid, and bound anthranilic acid, different from the existing metabolites, were detected by paper chromatography.