2014•Stem Cell ReportsOpen access
Human Intestinal Tissue with Adult Stem Cell Properties Derived from Pluripotent Stem Cells
Ryan Forster, Kunitoshi Chiba, Lorian Schaeffer, Samuel G. Regalado, Christine S. Lai, Qing Gao, Samira Kiani, Henner F. Farin, Hans Clevers, Gregory J. Cost, Andy Chan, Edward J. Rebar, Fyodor D. Urnov, Philip D. Gregory, Lior Pachter, Rudolf Jaenisch, Dirk Hockemeyer
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Abstract
(Stem Cell Reports 2, 838–852, June 3, 2014) An inadvertent production error occurred after the proof stage while setting Table 1 for print. As a result, the labels “GFP Negative,” “GFP Positive,” and “Total” were positioned incorrectly. We apologize for this mistake and provide a corrected version of Table 1 below. The online version has also been corrected.Table 1WIBR3-hESC Derived:Teratoma-derived organoidsaSix independent teratomas FACS sorted and assayed for organoid formation.TeratomasGFP NegativeGFP PositiveTotalNo. of organoids formedNo. of cells sortedCells forming organoids (%)No. of organoids formedNo. of cells sortedCells forming organoids (%)No. of organoids formedNo. of cells sortedCells forming organoids (%)Lgr5-GFP N-term01,0000231,0002.3∼50>50,000<0.1%Lgr5-GFP N-term01,71001011,7105.91∼50>50,000<0.1%Lgr5-GFP N-term01,6750211,6751.25∼50>50,000<0.1%Lgr5-GFP C-term04,2030384,2030.9∼50>50,000<0.1%Lgr5-GFP C-term06,6130816,6131.22∼50>50,000<0.1%Lgr5-GFP N-term06,046036,0460.05∼50>50,000<0.1%Organoid-rederived organoidsbThree organoids derived from three independent unsorted teratomas (dissociated, FACs sorted, and assayed for organoid formation).OrganoidsGFP NegativeGFP PositiveTotalNo. of organoids formedNo. of cells sortedCells forming organoids (%)No. of organoids formedNo. of cells sortedCells forming organoids (%)No. of organoids formedNo. of cells sortedCells forming organoids (%)Lgr5-GFP N-term01,500071,5000.47715,0000.05Lgr5-GFP N-term246,7000.361406,7002.096513,0000.5Lgr5-GFP N-term37,9600.04207,9600.261538,4500.04a Six independent teratomas FACS sorted and assayed for organoid formation.b Three organoids derived from three independent unsorted teratomas (dissociated, FACs sorted, and assayed for organoid formation). Open table in a new tab Human Intestinal Tissue with Adult Stem Cell Properties Derived from Pluripotent Stem CellsForster et al.Stem Cell ReportsJune 03, 2014In BriefHockemeyer, Jaenisch, and colleagues developed a method for deriving human intestinal organoid cultures from hPSCs. Isolating intestinal cells from hPSC-derived teratomas yields intestinal cultures similar to organoids isolated from primary tissue. Molecular and histological analyses demonstrate that hPSC-derived organoids comprise the major cell types of the intestinal epithelium, including adult stem cell-like cells. Transcriptional profiling reveals that hESC-derived organoids are highly similar to organoids isolated from primary tissue. Full-Text PDF Open Access
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