THE STUDY OF BACTERIOLOGICAL PROFILE AND ANTIBIOGRAM OF NEONATAL SEPTICEMIA

Septicemia accounts for a significant proportion of morbidity and mortality in the newborn and is therefore a major problem in pediatric practice worldwide.The objective of this study was to know the bacteriological profile and antibiogram of neonatal septicemia of NICU (Neonatal Intensive Care Unit) of CIMS.Under aseptic precautions, blood was drawn from 500 neonates with suspected septicemia and inoculated in biphasic media.Isolates obtained were identified as per standard protocol and antibiotic susceptibility was done by Kirby Bauer disc diffusion method as per CLSI (Clinical and laboratory standards institute) guidelines.A total number of 78(15.6%)patients had positive blood cultures.The most common pathogens isolated were Staphylococcus aureus (n=22, 28.20%) followed by Escherichia coli (n=19, 24.3%), Enterobacter spp (n=10, 12.82%), Coagulase negative Staphylococcus (n=9, 11.53%), Proteus spp.(n=8, 10.25%), Pseudomonas spp.(n=5, 6.41%), Acinetobacter spp (n=4, 5.12%), Klebsiella spp (n=1, 1.28%).The Gram negative organisms were more resistance to commonly used antibiotics like Cefotaxime, Ampicillin but highly sensitive to Imipenam.The Gram positive bacteria showed high resistance to Chloramphenicol, Penicillin but they were highly susceptible to Vancomycin and Gentamycin.As the Gram negative organisms were the most common isolates in neonatal septicemia, As there is increasing incidence of resistant strain in common isolates of neonatal septicemia.