The suspensor analysis and morphology of somatic embryo of the meristem culture of ginger
Rama Riana Sitinjak
Abstract
Rama Riana Sitinjak
Abstract
The aim of this research was to evaluate the somatic embryo of meristem culture of ginger (Zingiber officinale Rosc.) through morphological analysis. This research used descriptivee method. Friable embryogenic callus, obtained from meristem culture, was cultured on proliferation medium. Cultured tissue fragments containing several globular embryoids were observed by microscopy to follow development of ginger embryoids. Based at morphology analysis, at first week it grew into somatic embryo. Somatic embryo that rise from embriogenic cells were another contact via a route specific. The somatic embryo formed was consist of an apical region, a basal region and a suspensor region. On the fourth week, however, the morphology of somatic embryo was founded on the shape of globular. It’s consist of an apical region and a basal region.
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The aim of this research was to evaluate the somatic embryo of meristem culture of ginger (Zingiber officinale Rosc.) through morphological analysis. This research used descriptivee method. Friable embryogenic callus, obtained from meristem culture, was cultured on proliferation medium. Cultured tissue fragments containing several globular embryoids were observed by microscopy to follow development of ginger embryoids. Based at morphology analysis, at first week it grew into somatic embryo. Somatic embryo that rise from embriogenic cells were another contact via a route specific. The somatic embryo formed was consist of an apical region, a basal region and a suspensor region. On the fourth week, however, the morphology of somatic embryo was founded on the shape of globular. It’s consist of an apical region and a basal region.
Key concepts: Suspensor, Somatic embryogenesis, Meristem, Somatic cell, Embryo, Biology, Callus, Botany