2016•Nanomechanics Science and Technology An International JournalRequires access
CITRATE-STABILIZED NANOPARTICLES OF CeO2 STIMULATE PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO
Anton L. Popov, Artem M. Ermakov, I. V. Savintseva, И. И. Селезнева, Р. А. Полтавцева, Е. I. Zaraisky, A. M. Poltavtsev, А. А. Степанов, В. К. Иванов, Г. Т. Сухих
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Abstract
Application of mesenchymal stem cells (MSCs) is one of the most prospective approaches in recovery of damaged organs. Currently, several reasons underlie the limitations of wide clinical use of human MSCs, including their low proliferation rate in vitro. In account of this, searching for new safe and efficient proliferation inducers is an urgent task. In the present study, we investigated the influence of citrate-stabilized cerium oxide (CeO2) nanoparticles on the proliferative activity of human MSCs. Introduction of CeO2 nanoparticles in MSC culture was shown to result in an intensification of cell proliferation rate in a dose-dependent manner. The conducted analysis of the transcription profile of human MSCs in the presence of CeO2 nanoparticles confirmed an increased transcription level for mRNAs of genes responsible for proliferation and cell cycle, as well as suppression of apoptosis. The derived results clearly indicate that CeO2 nanoparticles can be used as a basis for developing highly efficient inexpensive supplement for cell culturing in vitro.