Absorbance correction method for the simultaneous estimation of hydrochlorothiazide, telmisartan and amlodipine besylate in API andcombined tablet dosage formulation by UV spectrophotometry

A new, simple, sensitive and accurate UV ‐ spectrophotometric absorption correction method has been developed for simultaneous determination of amlodipine besylate, telmisartan and hydrochlorothiazide in combined tablet dosage form. The wavelengths selected for the analysis were 365 nm, 250 nm and 335 nm for amlodipine besylate, telmisartan and hydrochlorothiazide respectively. Beer’s law obeyed the concentration range of 10 - 60 μg/ ml, 4 – 20 μg/ ml and 20 - 100 μg/ ml for amlodipine besylate, telmisartan and hydrochlorothiazide, respectively. Methanol and distilled water were used as solvents. The percentage recovery was found in the range of 98.9% to 101.6% for AMB, 99.6% to101.3% for TEL and 99.8 % to 101.2% for HCT. The developed method was validated statistically. The % RSD value was found to be less than 2. Thus the proposed method was simple, precise, economic, rapid, accurate and can be successfully applied for simultaneous determination of amlodipine besylate, telmisartan and hydrochlorothiazide in combined tablet dosage form.