Effects of Medium Components on Shoot Formation from Cultured Tuber Discs of Potato1

Abstract The effects of the components of Lam's 1977 potato nutrient medium on adventitious shoot formation were systematically evaluated. A low concentration (0.03 mg/liter) of naphthaleneacetic acid (NAA) stimulated shoot initiation and increased survival of tuber discs of potato ( Solanum tuberosum L.). Indoleacetic acid (IAA), however, did not affect shoot initiation in the concentration range from 0 to 10 mg/liter. Both 6-benzyl-amino purine (BA) at 0.3, 1.0, and 3.0 mg/liter and N 6 -(4-hydroxy-3-methyl-but-2-enylamino)-purine (zeatin) at 0.3 and 1.0 mg/liter stimulated adventitious shoot formation while N 6 -furfurylaminopurine (kinetin) had no effect. Sucrose was essential for shoot formation with a concentration of 30 g/liter being optimal. Although not essential, i -inositol enhanced the initiation of adventitious shoots at concentrations of 60 and 100 mg/liter. Casein hydrolysate and additional inorganic phosphate had no promotive effect on shoot formation and adenine sulfate was inhibitory at all concentrations examined.