2013Journal of natural product and plant resourcesRequires access

Phytochemical Screening and Spectroscopic Determination of Total Phenolic andFlavonoid Contents of Eclipta Alba Linn

Mohd Yousuf Malla, Manik Sharma, RC Saxena Mohd Iqbal Mir, Abrar Hussain Mir, Showkat Hussain Bhat

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Abstract

This research aims to detect the Phytochemical Screening and in-vitro total phenolic and flavonoids contents of chloroform and methanolic extracts of Eclipta Alba. All the main phytoconstituents were present in methanolic extract as compared to chloroform extract. The total phenolic content and total flavonoid content were determined by Folin–Ciocalteu and aluminum chloride methods respectively. The total phenolic contents of methanolic extract (269.5±0.5mg/g) and chloroform (182±0.5 mg/g), were expressed as Gallic acid equivalents The flavonoid contents were found to be (139.5±0.5mg/g) and (85.666±0.2886mg/g) in methanol and chloroform extracts respectively using rutin equivalents. The results are in concurrence with the phytochemical properties present in the plant.

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What this paper is about

This research aims to detect the Phytochemical Screening and in-vitro total phenolic and flavonoids contents of chloroform and methanolic extracts of Eclipta Alba. All the main phytoconstituents were present in methanolic extract as compared to chloroform extract. The total phenolic content and total flavonoid content were determined by Folin–Ciocalteu and aluminum chloride methods respectively. The total phenolic contents of methanolic extract (269.5±0.5mg/g) and chloroform (182±0.5 mg/g), were expressed as Gallic acid equivalents The flavonoid contents were found to be (139.5±0.5mg/g) and (85.666±0.2886mg/g) in methanol and chloroform extracts respectively using rutin equivalents. The results are in concurrence with the phytochemical properties present in the plant.

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Available abstract

This research aims to detect the Phytochemical Screening and in-vitro total phenolic and flavonoids contents of chloroform and methanolic extracts of Eclipta Alba. All the main phytoconstituents were present in methanolic extract as compared to chloroform extract. The total phenolic content and total flavonoid content were determined by Folin–Ciocalteu and aluminum chloride methods respectively. The total phenolic contents of methanolic extract (269.5±0.5mg/g) and chloroform (182±0.5 mg/g), were expressed as Gallic acid equivalents The flavonoid contents were found to be (139.5±0.5mg/g) and (85.666±0.2886mg/g) in methanol and chloroform extracts respectively using rutin equivalents. The results are in concurrence with the phytochemical properties present in the plant.

Key concepts: Phytochemical, Chloroform, Gallic acid, Rutin, Flavonoid, Chemistry, Traditional medicine, Phenols

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