Utility of Random Amplified Polymorphic DNA (RAPD) In

Forensic entomology is the study of insects found around the crime scene and are helpful for the estimation of time elapsed since death of a person i.e., post mortem interval (PMI). There are number techniques available for the identification of insect species and Random Amplified Polymorphic DNA (RAPD) is one of those. RAPD is a useful technique designed for the detection of sequence polymorphism in organisms where previous nucleotide sequence data is not available. RAPDs are based on random priming using short primers of arbitrary sequence that act as both forward and reverse primers in the PCR reaction.