Changes of kinetic parameters in bacterial oxidation ofelemental sulfur
Martin Mandl, Blanka Omesová Pokorná, Šárka Bořilová, Pavla Češková, Oldřich Janiczek, Romana Dohnalová
Abstract
Martin Mandl, Blanka Omesová Pokorná, Šárka Bořilová, Pavla Češková, Oldřich Janiczek, Romana Dohnalová
Abstract
Biooxidation of elemental sulfur plays an important role in biohydrometallurgy and spontaneous biogeochemical processes in sulfide deposits (sulfide wastes, abandoned ore mines). The purpose of this study was to investigate a correlation among growth conditions, physiological factors and selected kinetic constants of elemental sulfur oxidation by Acidithiobacillus ferrooxidans. The values of maximum sulfur oxidation rate (v) and growth yield on sulfur (Y) were determined from data obtained in 73 batch cultures. The changes of the apparent Michaelis constant for sulfur (Km) were investigated in resting cells.
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Biooxidation of elemental sulfur plays an important role in biohydrometallurgy and spontaneous biogeochemical processes in sulfide deposits (sulfide wastes, abandoned ore mines). The purpose of this study was to investigate a correlation among growth conditions, physiological factors and selected kinetic constants of elemental sulfur oxidation by Acidithiobacillus ferrooxidans. The values of maximum sulfur oxidation rate (v) and growth yield on sulfur (Y) were determined from data obtained in 73 batch cultures. The changes of the apparent Michaelis constant for sulfur (Km) were investigated in resting cells.
Key concepts: Sulfur, Sulfide, Biogeochemical cycle, Chemistry, Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, Yield (engineering), Sulfur cycle