Growth and study of micro-crystalline calcium oxalate monohydrate crystals by agar gel system

Various crystals are found in human urine. Of those, the calcium oxalate is one of the principle components of urinary stone. It is found in three different hydrate forms, Calcium oxalate monohydrate (COM), calcium oxalate dihydrates (COD) and calcium oxalate trihydrates (COT).Calcium oxalate monohydrate (CaC2O4.H2O) is most thermodynamically stable form of calcium oxalate and occupies the biggest proportion of all the urinary stones.The growth of calcium oxalate monohydrate crystals is achieved in agar-agar gel by single diffusion and double diffusion gel techniques. Result shows the white microcrystalline precipitation was formed in both techniques.The grown crystal were characterized by optical microscopy, Fourier Transform Infrared Spectroscopy (FTIR), powder X-ray diffraction (XRD) and thermogravimetric analysis (TGA). Morphology of COM crystals were reveled by Optical microscopy, whereas functional groups were confirmed by FTIR analysis. Crystalline nature was confirmed by XRD. In third stage of decomposition, sample was converted as a residue of calcium oxide after releasing of CO2 which confirmed the structure of COM.