2003•Unpublished venueRequires access
The protein spectrum changes of two silkworm (Bombyx mori L.)hybrids during the development
Pavel Hyršl, Vladimír Šimek
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Abstract
The aim of this study was to analyse the protein spectrum of
the haemolymph of the silkworm (Bombyx mori L., Lepidoptera,
Bombycidae) during the development. We used two monovoltine
bulgarian hybrids (Super1 x Vratza 35)x(Tv x Merefa2) and AS x
KK. The haemolymph samples were collected every day of the last
(5th) larval instar (8 days) and in the stage of prepupa and
pupa. The samples were subjected to polyacrylamide gradient gel
electrophoresis (SDS-PAGGE) and protein molecular weight was
determined (Molecular Analyst software). We found 16 - 23
protein fractions in the range of 6.5 200 kDa. The main
observed protein groups were: 200 kDa lipophorin, storage
proteins in the range of 80 kDa and 30 kDa polypeptides. We
demonstrated significant quantitative and qualitative changes
in the haemolymph protein spectrum during development
(continuous increase in the level of 30 kDa polypeptides and
storage proteins during 5th instar) as well as differences in
protein spectrum between males and females. No significant
differences in protein spectrum were apparent between the two
hybrids.