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2017•Advanced Techniques in Biology & MedicineRequires access

Sandwich ELISA for Circulating Myeloperoxidase- and Neutrophil Elastase-DNA Complexes Released from Neutrophil Extracellular Traps

Hideki Kano, Muhammad Aminul Huq, Masanobu Tsuda, Hiroshi Noguchi, Naoshi Takeyama

Open publisher page 49 citations

Abstract

Neutrophil extracellular traps (NETs) are DNA scaffolds released by activated neutrophils that contain enzymes from neutrophil granules, such as myeloperoxidase (MPO), neutrophil elastase (NE) and cathepsin-G. NETs are produced by neutrophils in response to various stimuli, including invasion of pathogenic microorganisms. Here we describe a new ELISA method for quantifying the circulating levels of MPO- and NE-associated DNA in human plasma.

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What this paper is about

Neutrophil extracellular traps (NETs) are DNA scaffolds released by activated neutrophils that contain enzymes from neutrophil granules, such as myeloperoxidase (MPO), neutrophil elastase (NE) and cathepsin-G. NETs are produced by neutrophils in response to various stimuli, including invasion of pathogenic microorganisms. Here we describe a new ELISA method for quantifying the circulating levels of MPO- and NE-associated DNA in human plasma.

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Available abstract

Neutrophil extracellular traps (NETs) are DNA scaffolds released by activated neutrophils that contain enzymes from neutrophil granules, such as myeloperoxidase (MPO), neutrophil elastase (NE) and cathepsin-G. NETs are produced by neutrophils in response to various stimuli, including invasion of pathogenic microorganisms. Here we describe a new ELISA method for quantifying the circulating levels of MPO- and NE-associated DNA in human plasma.

Key concepts: Neutrophil extracellular traps, Myeloperoxidase, Neutrophil elastase, Elastase, Neutrophile, Chemistry, Immunology, DNA

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Sandwich ELISA for Circulating Myeloperoxidase- and Neutrophil Elastase-DNA Complexes Released from Neutrophil Extracellular Traps — Research Paper | ScholarLens