2010•Unpublished venueRequires access

ANTIBACTERIAL ACTIVITY OF TRUNK BARK OF ALSTONIA SCHOLARIS

Altaf Hussain, Kamaruz Zaman, M.D. Ramteke

Open publisher page 27 citations

Abstract

The in vitro antibacterial activity of methanol, aqueous and total alkaloid extracts from the trunk bark of Alstonia scholaris (L.) R.Br (Apocynaceae), growing in north‐east India was evaluated against two Gram‐positive bacteria including Bacillus subtilis and Streptococcus pyogens and four Gram‐ negative bacteria, Escherichia coli, Klebsiella pneumoniae, Pseudomonous aeruginosa and Proteus mirabilis using disk diffusion method. All extracts showed varying degrees of inhibitory activity against all bacteria. Aqueous extract was found very active against both Gram‐positive and Gram‐ negative bacteria. The extracts showed antibacterial activity against two Gram‐positive bacteria and one Gram‐negative in a dose dependent manner other two extract was found less active in comparison to aqueous extract. Methanol extract was found active against both Gram‐positive and Gram‐negative bacteria without showing dose dependency. Total alkaloidal extract was found only active against gram‐negative bacteria. Comparatively Gram‐positive bacteria were more sensitive to the extracts, except total alkaloid extract.

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What this paper is about

The in vitro antibacterial activity of methanol, aqueous and total alkaloid extracts from the trunk bark of Alstonia scholaris (L.) R.Br (Apocynaceae), growing in north‐east India was evaluated against two Gram‐positive bacteria including Bacillus subtilis and Streptococcus pyogens and four Gram‐ negative bacteria, Escherichia coli, Klebsiella pneumoniae, Pseudomonous aeruginosa and Proteus mirabilis using disk diffusion method. All extracts showed varying degrees of inhibitory activity against all bacteria. Aqueous extract was found very active against both Gram‐positive and Gram‐ negative bacteria. The extracts showed antibacterial activity against two Gram‐positive bacteria and one Gram‐negative in a dose dependent manner other two extract was found less active in comparison to aqueous extract. Methanol extract was found active against both Gram‐positive and Gram‐negative bacteria without showing dose dependency. Total alkaloidal extract was found only active against gram‐negative bacteria. Comparatively Gram‐positive bacteria were more sensitive to the extracts, except total alkaloid extract.

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Available abstract

The in vitro antibacterial activity of methanol, aqueous and total alkaloid extracts from the trunk bark of Alstonia scholaris (L.) R.Br (Apocynaceae), growing in north‐east India was evaluated against two Gram‐positive bacteria including Bacillus subtilis and Streptococcus pyogens and four Gram‐ negative bacteria, Escherichia coli, Klebsiella pneumoniae, Pseudomonous aeruginosa and Proteus mirabilis using disk diffusion method. All extracts showed varying degrees of inhibitory activity against all bacteria. Aqueous extract was found very active against both Gram‐positive and Gram‐ negative bacteria. The extracts showed antibacterial activity against two Gram‐positive bacteria and one Gram‐negative in a dose dependent manner other two extract was found less active in comparison to aqueous extract. Methanol extract was found active against both Gram‐positive and Gram‐negative bacteria without showing dose dependency. Total alkaloidal extract was found only active against gram‐negative bacteria. Comparatively Gram‐positive bacteria were more sensitive to the extracts, except total alkaloid extract.

Key concepts: Bacteria, Proteus mirabilis, Apocynaceae, Gram-positive bacteria, Gram-negative bacteria, Antibacterial activity, Bark (sound), Biology

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