2015•Unpublished venueRequires access
Variables affecting the recombinant phytase production from Escherichia coli DH5a
Rafidah Mohd Ariff, Mohd Yazid Abdul Manap, Aini Ideris, Azhar Kassim, Abd-El Aziem Farouk, Anis Shobirin Meor Hussin
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Abstract
Screening for the important variables is part of the optimization study of enzyme production. It is important indetermining the influential variables that give a significant effect on the enzyme production. Thus, the objective of this study was to determine variables that are effecting the recombinant phytase production from Escherichia coli DH5±. Through the application of the Fractional Factorial Design, the variables of seed age, inoculum level, time of induction, L-arabinose concentration and post-induction time were highly significant for the recombinant phytase production as their p-values were less than 0.05.Thus, minimal changes of this factor mat effect recombinant phytase production from E. coli DH5±.