2005•Fungal Genetics ReportsOpen access

An effect of homology length on gene disruption in Neurospora crassa

Yoshinobu Nakanishi, Chizu Ishii, H. Inoue

Abstract

For targeted gene disruption in wild-type Neurospora crassa, 1000-bp of homologous sequences on either side of the cassette used for disruption is sufficient to give more than 10 % homologous recombination. We report here that varying the length of homology on each side seems to have different effects on the homologous recombination frequency.

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Key concepts: Neurospora crassa, Homologous chromosome, Homologous recombination, Homology (biology), Genetics, Gene, Recombination, Biology