2004•Unpublished venueRequires access
Identification and mapping of resistance genes to powderymildew in barley.
Jana Řepková, Zdenka O. Kyjovska, Pavel Lı́zal, Antonín Dreiseitl, Ahmed Jahoor
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Abstract
The introduction of resistance genes to powdery mildew
(Blumeria graminis f. sp. hordei) present in two accessions
(PI354949 and PI466495) of wild barley (Hordeum vulgare subsp.
spontaneum) into H. vulgare was performed by the cross with the
variety Tiffany. Powdery mildew resistance tests on plants of
F2 generation revealed the segregation ratio 15 : 1 in Tiffany
x PI354949 and 3 : 1 in Tiffany x PI466495, which indicated
presence of two and one dominant resistance genes,
respectively. To identify individual R genes, technology of
microsatellite DNA markers and linkage recombination analysis
was applied. One of resistance genes in PI354949 was determined
to be located in Mla locus linked with Bmac0213 (25.0 cM in
distal position) on chromosome 1H and the other on chromosome
7H linked with Bmag0507 (14.0 cM in proximal position). In
PI466495, R gene was linked with Bmac0213 (8.4 cM in distal
position) and it is also located in Mla locus. The perspective
aim of this work is fine-mapping of the promising gene located
on 7H chromosome and identification of tightly linked DNA
markers for marker assisted selection.