Clinical reaserch on DCTAA and CIK from stem in treating patients of moderate and advanced stage lung cancer

Objective To observe the treatment effects in 48 cases of advanced lung cancer patients,with the immune therapy of the dendritic cells loading of tumor autologous antigen (DCTAA) combining with the cells induced factor of the killer cells (CIK) from the matched umbilical cord blood cells. Methods The peripheral blood mononuclear cell (PBMC) from the matched umbilical cord blood cells was seperated, and induced to CIK and DC with some cytokines in vitro, such as CD3McAb, IL-2, IFN-γ IL-1α, etc. After 12 to 15 days, the amplified CIK cells obtained were obtained, with the strict quality control, infused the CIK cells to the patients body back in six times, about (5-8)×109 CIK cells in each time. In the fifth day of the cultivation, DETAA cells were loaded and DCTAA cells were collected in the eighth day, and then hypodermic injection was done. The patient' s general situation after the immune treatment was observed, such as the size of the tumors, clinical symptom score, the quality of life and immune indexes. Karnofsky score, weight, toxic side effects and the patient's survival were also studied. Results In the 48 cases with the DCTAA-CIK treatment, complete remission (CR), partial remission (PR) was 37 cases, the overall remission rate was 77.1％. The improvement rate of clinical symptom scores was from 78.9 ％ to 84.7 ％, the increasing rate of Karnofsky score was 89.6 ％ (43/48). 1-year survival reached to 80.6 ％. There were significant difference in little toxic side effects(P ＜ 0.01). The proportion of CD3, CD4 and NK cells in peripheral blood cells increased significantly (P ＜ 0.01) after DCTAA-CIK cells treatment[(42.21±6.12)％, (24.42±3.01)％, 0.99±0.34, (24.98±3.02) ％; (71.58±7.64) ％, (37.25±2.13) ％, 1.62±0.45, (35.23±4.11) ％](t = 6.34, 5.67, 0.25, 4.43, P ＜0.01).Conclusion The DCTAA-CIK immune therapy is benefit for advanced lung cancer, not only improve the immune function but also ameliorate the clinical symptoms. Key words: Dendritic cells;  Vaccine;  Killer cells;  Cytokines;  Antigens, neoplasms;  Flow cytometry;  Carcinoma, non-small-cell lung;  Immunotherapy, adoptive