DETERMINATION OF LUPEOL, B-SITOSTEROL AND QUERCETIN FROM ETHYL ACETATE EXTRACT OF RHIZOPHORA MUCRONATA BARK BY HPTLC TECHNIQUE

Rhizophora mucronata bark is tropical plant growing in saline water and maintains the ecosystem of the environment. In India the plant was very much used in leather industry because of its rich tannin content. Quantitative determination of lupeol , β–sitosterol, and quercetin was done by HPTLC using toluene, ethyl acetate and formic acid in different ratio. The detection and quantification was performed at 296 nm, 300 nm & 320 nm respectively. The correlation coefficient obtained for the linearity was 0.98526 for β–sitosterol, 0.98966 for lupeol, and 0.99526 for quercetin. The amount of lupeol, β–sitosterol, and quercetin was found to be 3.34 µg/ mg, 3.25 µg/ mg and 2.352 µg/ mg of ethyl acetate extract of R mucronata bark.