2013•Unpublished venueRequires access
Construction of stably transfected human cell lines for toxicological and environmental applications
Aneta Novotná
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Abstract
PhD. thesis is focused on construction of stable transfected cell lines derived from human cancer cell lines. First part of thesis is focused on construction and characterization of stable gene reporter human cell lines, generated from HepG2 and HeLa cells, allowing the assessment of transcriptional activities of the human glucocorticoid receptor and the human aryl hydrocarbon receptor. The second part is interested in construction and characterization of hepatocyte-like human cell lines, generated from the commercial human cancer cell line HepG2 stably transfected with PGC-1? and HNF4? vectors with the aim to improve the hepatospecific functions.