Effects of treatment with different fluids on systemic inflammatory response in newborn rabbits with septic shock

Objective To evaluate the effects of treatment with different fluids on systemic inflammatory response in newborn rabbits with septic shock. Methods One hundred New Zealand white rabbits （3-5 days, 45- 70 kg） were randomly divided into 5 groups （n = 20 each）： group I sham operation （group S）; group II sepsis （group CLP）; group m lactated Ringer＇s solution （group R）; group IV 6% HES （group HES） and group V （group R ＋ HES）. The animals were anesthetized with intraperitoneal ketamine and midazolam. Sepsis was induced by cecal ligation and puncture （CLP）. The animals in group R and HES received lactated Ringer＇s solution and 6% hydroxyethyl starch （HES） 30 ml/kg infused iv over 1 h respectively immediately after CLP, while group R ＋ HES received Ringer＇s solution 20 ml/kg and 6% HES 10 ml/kg. No fluid was infused in groups S and CLP. Carotid artery and jugular vein were cannulated for MAP monitoring, blood sampling and drug and fluid administration. MAP was recorded immediately （T1） and at 0.5, 1, 2, 4, 8 h （ T2-6 ） after CLP. Arterial blood samples were collected at T6 for determination of plasma IL-6 and IL-10 concentrations. Results Compared with those at T1 , MAP decreased significantly after CLP at T4-6 in group CLP and at T5-6 in group R but hypotension did not develop after CLP in group HES and R ＋ HES. Plasma IL-6 concentration was significantly higher in group CLP and R than in group S. There was no significant difference in plasma IL-6 concentration between group S and group HES and R ＋ HES. Plasma IL-10 concentration was significantly lower in group CLP and higher in group HES than in group S. There was no significant difference in plasma IL-10 concentration between group S and group R and R ＋ HES. Plasma IL-6 concentration was significantly lower, while plasma IL-10 concentration was significantly higher in group R ＋ HES and HES, and plasma IL-10 concentration was significantly higher in group R than in group CLP （ P 〈 0.05 ）. Conclusion Ringer＇ s solution ＋ 6 % HES （2 ： 1 ） is better than Ringer＇ s solution or 6% HES infused iv alone in maintaining the balance between pro-and anti-inflammatory cytokines in newborn rabbits with septic shock. Key words: Fluid therapy;  Shock, septic;  Systemic inflammatory response syndrome