Isolation, Characterization and Antibacterial Activity of Crude and Purified Saponin Extract from Seeds of Soyabean

In the present investigation, the inhibitory effect of crude and purified saponin extract from Glycine max was evaluated against some pathogenic bacterial strains (gram positive and gram negative) using agar disc diffusion method. Agar well diffusion method was adapted to determine antibacterial activity against all the test microorganisms. Zone of inhibition of the crude and pure extracts were tested. Among all the test pathogens Vibrio harveyi was found most susceptible with zone of inhibition 16 mm. to the crude and purified saponin extract. The present study successfully isolated and characterized the single unit of Glycine max saponin with 27-kDa units. It also reveals the least susceptibility of the microorganisms towards the saponin isolated from seeds of Glycine max.