Identification of New Fungi Isolated from Euonymus spp. in Guilan Province

Euonymus spp. (L.) are some of the most important ornamental plants. Fungal plant pathogens of ornamental plants are the most important factors causing damage. In this research two pathogenic fungal species were isolated from naturally infected Euonymus species and identified. In order to isolation of fungi from disease tissues, the obtained samples were cultured on potato dextrose agar medium. Isolates were cultured due to sporulation on water agar medium. Morphological characters of isolates were studied in order to identify the taxonomy. According to the results, isolates were belonged to Phoma sp. and Colletotricum gloeosporioides. Pathogenicity test of isolates was done in desiccators, and revealed the pathogenicity levels of these fungi and their ability to cause leaf blight on Euonymus spp. This reaction occurred as complete random design (CRD) with eight treatment and three replications. Based on the variance analysis, there was not any significant difference in the disease rating of studying fungi. But based on the sizes and types of the spots appeared on the Euonymus spp. and Horsfall-Barratt system, plants were more affected by the Phoma sp. compared withC. gloeosporioides., and its disease rating was higherand plants showed less tolerance.