Разработка метода и оценка эффективности долгосрочного хранения клеток костного мозга

It is known that the biological objects are stored frozen for a long time. The process of bone marrow cryopreservation without cryoprotectants is very unfavorable for the cells, it causes the use of cryoprotectants during freezing. Action cryoprotectants reduce the negative effects of freezing and thawing, and stores the cells prior to transplantation, but incorrectly selected cryoprotectant may adversely affect the safety and the level of energy processes in the cells. The effect of cryoprotectants and freezing-thawing on the structural and morphological status of dogs bone marrow. Final concentrations of cryoprotectants used: DMSO 10 %, 7 %, 5 %, PEO-400 10 %, 15 %, 20 %, glycerol 10 %, 20 %, 30 %. These results characterize 7 % DMSO as a cryoprotectant most effective for a lot of types of bone marrow cells of dogs, especially for cells in the early stages of differentiation. DMSO stored for more than 80 % of bone marrow cells of dogs. PEO 400 is able to maintain cells during cryopreservation, but the final stages of the process, amount of cells is greatly reduced. Glycerol in the concentrations tested, was less effective of the studied cryoprotectants.