2016•Molecular TherapyOpen access
95. Incorporation of AAV Serotype 2 Viral Protein 2 into Other AAV Serotype Capsids
Daniel Zhang, Jun Li, Yinglu Feng, Adam Snowden, Georgy Aslanidi, Nicholas Muzyczka, Arun Srivastava, Changquan Ling, Chen Ling
Open full text 0 citations
Abstract
It was previously documented that large ligands inserted after residue 138 in the adeno-associated virus serotype 2 (AAV2) viral protein 2 (VP2) is tolerant to produce nearly wild-type levels of recombinant AAV2 (rAAV2) vectors (J Virol. 78(12):6595-609, 2004). Furthermore, insertion of enhanced green fluorescent protein (EGFP) into VP2 resulted in a rAAV2 particle, whose trafficking could be temporally monitored using confocal microscopy. Here, we examined whether AAV2 VP2 (2VP2) could be incorporated into other AAV serotype capsids during viral production.
Open-access reader