ISOLATION AND CHARACTERIZATION OF BIOACTIVE STREPTOMYCES FROM MANGROVE ECOSYSTEM OF MACHILIPATNAM, KRISHNA DISTRICT, ANDHRA PRADESH

ABSTRACTObjective: The aim of the present study was to isolate, identify, and analyze the taxonomic characteristics of the potent actinobacterial strains VJSY-2and VJSY-3 isolated from mangrove soils of Gilakaladindi, Machilipatnam, Krishna District of Andhra Pradesh.Methods: Soil samples pretreated with calcium carbonate were used for isolation of potent actinobacterial strains. Identification of the strains wascarried out by studying the cultural, morphological, biochemical, and physiological characteristics. The phylogenetic study of the strains was carriedout by employing 16S r DNA sequence-based analysis. Phylogenetic tree was constructed using the molecular evolutionary genetic analysis softwareversion 6.Results: Based on the polyphasic taxonomic studies, the potent strains belong to Streptomyces genus. The bioactive metabolites produced by the strainswere active against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Bacillus megaterium), Gram-negative bacteria (Xanthomonascampestris, Pseudomonas aeruginosa, and Escherichia coli), and fungi (Aspergillus niger, Fusarium solani, Fusarium oxysporum, and Candida albicans).Conclusion: The results of the experiment showed that the crude ethyl acetate extract of the strains VJSY-2 and VJSY-3 showed significant antimicrobialpotential; hence, they can be used for isolation of compounds with pharmaceutical importance.Keywords: Mangrove Actinobacteria, Streptomyces, Bioactive metabolites.