Effects of pomegranate juice as diluent supplementation on sperm quality during liquid storage of chicken semen

in vitro storage method for roosters' semen, an experiment w as conducted to study the influence of inclusion pomegranate juice (PJ) into semen diluent on semen quality during liq uid storage for up to 72 h. A total of 60 White Leghorn roosters, 40 weeks of age, ran domly divided into 6 treatment groups (10 males each) were used in this study. Tre atment groups were as follows: T1 = fresh semen, T ji 2 diluent (AD2D) alone, T3 -T6 = semen diluted 1:2 with AD2D supplemented l, 5 ml or 7 ml of PJ / 100 ml of diluent, respecti vely. Semen samples were assessed after ˚C for 12 h, 24 h or 36 h as regards mass activity, individual motility and percentages of dead spermatozoa, abnormal spermatozoa and acros omal abnormalities. Results revealed that supplemen ting the diluent of roosters semen with PJ (T3, T4, T5 and T 6) and then store it for different storage periods (12 h, 24 h or 36 h) resulted in significant (p < 0.05) improve ment in spermatozoa motility, v iability, morphology and acrosomal integrity in comparison with control grou p (T1). Moreover, T5 and T6 surpasses other treatme nts with respect to these semen characteristics, while there were no significant differences between T2, T3 and T4 semen traits included in this study. In conclusion, the substitution of AD2D diluent composition with PJ significantly improves quality of roosters semen that in vitro stored for up to 36 h. Furthermore, the positive effect of PJ observed in this study ma y be due to enhanced sperm resistance to lipid pero xidation that storage of avian semen. Pomegranate juice, semen quality , liquid storage, chicken semen storage method for roosters' semen, an experiment w as conducted to study the influence of inclusion pomegranate juice (PJ) into semen diluent on semen quality during liq uid domly divided into 6 treatment groups (10 males each) were used in this study. Tre atment groups were as follows: T1 = fresh semen, T 2 = T6 = semen diluted 1:2 with AD2D supplemented l, 5 ml or 7 ml of PJ / 100 ml of diluent, respecti vely. Semen samples were assessed after in vitro ˚C for 12 h, 24 h or 36 h as regards mass activity, individual motility and percentages of dead omal abnormalities. Results revealed that supplemen ting the diluent of roosters semen with PJ (T3, T4, T5 and T 6) and then store it for different storage periods (12 h, 24 h iability, morphology and acrosomal integrity in comparison with control grou p (T1). Moreover, T5 and T6 surpasses other treatme nts with respect to these semen characteristics, while there were no significant differences between T2, T3 and T4 semen traits included in this study. In conclusion, the substitution of AD2D diluent composition stored for up to 36 h. Furthermore, the y be due to enhanced sperm resistance to lipid pero xidation that