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DPPH FREE RADICAL SCAVENGING ACTIVITY OF PHENOLIC COMPOUNDS IN ARGEMONE MEXICANA LINN.

Rahul Sharma, Ankita Yadav, Richa Bhardwaj

Abstract

The present study was aimed to evaluate the total phenolic contents (TPC), total flavonoid contents (TFC) and the antioxidant efficacy of methanolic extracts of different plant parts (leaves, stems, roots, flowers and fruits) of Argemone mexicana. The results showed significant variation in the total phenolic and flavonoid content and antioxidant potential among the different plant parts. The highest total phenolic (23.5mg GAE/gdw) and flavonoidal content (34.5mg QE/gdw) was recorded in flowers. Antioxidant evaluation of A.mexicana was carried out using 1, 1-diphenyl-2picrylhydrazyl radical (DPPH) free radical scavenging assay and exhibited considerable antioxidant potential and showed good correlation with the total phenolic and total flavonoidal content. The highest radical scavenging effect was observed in flowers of A. mexicana with IC50 = 23.75µg/ml. The results of the study showed that A.mexicana possess significant antioxidant activity. Owning to these results, the plant has the potential to be used as a medicine against the diseases caused by free radicals.

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Key concepts: DPPH, Flavonoid, Antioxidant, Chemistry, Scavenging, Food science, Radical, Antioxidant capacity