Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells

// Hua Guo 1, * , Guowen Hu 1, * , Qing Yang 2 , Pei Zhang 1 , Wei Kuang 1 , Xingen Zhu 1 , Lei Wu 1 1 Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China 2 Department of Respiratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China * These authors have contributed equally to this work Correspondence to: Lei Wu, email: doctorleiming@163.com Xingen Zhu, email: Zhuxg1965@126.com Keywords: qRT-PCR, quantitative real-time PCR; lncRNA, long non-coding RNA (lncRNAs); CCK-8, cell counting kit-8 Received: September 14, 2016 Accepted: October 14, 2016 Published: November 09, 2016 ABSTRACT Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) is commonly investigated in a number of cancers. However, little is known of its expression and biological function in glioma biology. In the current study, we used quantitative real-time PCR (qRT-PCR) to determine the expression of CCAT2 in glioma tissues. We found that expression of CCAT2 was up-regulated in glioma tissues and significantly correlated with the advanced tumor stage (III/IV). Functional assays in vitro and in vivo demonstrated that knockdown of CCAT2 could inhibit proliferation, cell cycle progression and migration of glioma cells. Further analysis indicated the effect of CCAT2 knockdown on glioma cell phenotype through inhibiting Wnt/β-catenin signal pathway activity. Thus, our study provides evidence that CCAT2 may function as a potential biomarker for glioma.