Autodissemination of Insect Growth Regulator, Methoprene, with Two Formulations AgainstAedes albopictus

Recent autodissemination studies have popularly favored pyriproxyfen, but methoprene is already established and widely used in control programs. This study demonstrated that methoprene could also autodisseminate through contaminated gravid females. Arenas of oviposition cups were arranged around methoprene-contaminated bait stations. Gravid female Aedes albopictus were released to allow oviposition. All cups were then removed and tested in an insect growth regulator bioassay. Liquid formulations did not result in adequate autodissemination among treatment groups. Granular formulations pulverized into a fine powder resulted in successful autodissemination. Emergence inhibition was recorded as high as 85% in individual assays. Pooled inhibition across the granular trials was 56.7%. Methoprene has benefits similar to pyriproxyfen use and has the added advantage of already being established and readily available in existing mosquito control programs throughout the USA. Methoprene is a good candidate for continued investigation of autodissemination.