1978•Zeitschrift für Naturforschung COpen access

A Rapid Procedure for the Purification of Ferredoxin from Spinach Using Polyethyleneimine

Rolf K. Thauer, H. Schirrmacher, W. Schymanski, Peter Schönheit

Abstract

Abstract A rapid procedure for the purification of ferredoxin from spinach is described, which is based on the finding that ferredoxins can be precipitated with polyethyleneimines from acetone containing solutions. The ferredoxin was obtained in high yields (25-35 mg/kg spinach) and high purity (A420/A275= O.47) within less than 8 hours.

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Available abstract

Key concepts: Ferredoxin, Spinach, Acetone, Chemistry, Chromatography, Biochemistry, Enzyme