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Deteksi Vibrio harveyi menggunakan primer hemolisin pada benur udang windu Penaeus monodon Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon

Irma Suriyani, Ince Ayu, Ilmiah Kuruseng

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Abstract

This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic Vibrio on tiger shrimp post-larvae (PL) exposed under different exposure times in media inoculated with Vibrio harveyi. The PL of tiger shrimp were infected with 10 6 cfu/mL of V. harveyi by immersion method for three, six, 12, 24, 48 and 72 hours. The presence of hemolisin genes was detected by PCR techniques. The electrophoresis detected narrow hemolysin genes after PL were exposed for three and six hours. Clear visible bands of DNA Vibrio were observed for 12 hours of exposure. In contrast, no detected hemolysin gene of Vibrio was observed for PL exposed within 24, 48, and 72 hours. The rapid detection on Vibrio pathogenic for tiger shrimp PL should be conducted within three to 12 hours of exposure. No recommendation in utilizing this rapid detection for tiger shrimp PL exposed beyond 12 hours of V. harveyi.

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What this paper is about

This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic Vibrio on tiger shrimp post-larvae (PL) exposed under different exposure times in media inoculated with Vibrio harveyi. The PL of tiger shrimp were infected with 10 6 cfu/mL of V. harveyi by immersion method for three, six, 12, 24, 48 and 72 hours. The presence of hemolisin genes was detected by PCR techniques. The electrophoresis detected narrow hemolysin genes after PL were exposed for three and six hours. Clear visible bands of DNA Vibrio were observed for 12 hours of exposure. In contrast, no detected hemolysin gene of Vibrio was observed for PL exposed within 24, 48, and 72 hours. The rapid detection on Vibrio pathogenic for tiger shrimp PL should be conducted within three to 12 hours of exposure. No recommendation in utilizing this rapid detection for tiger shrimp PL exposed beyond 12 hours of V. harveyi.

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Available abstract

This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic Vibrio on tiger shrimp post-larvae (PL) exposed under different exposure times in media inoculated with Vibrio harveyi. The PL of tiger shrimp were infected with 10 6 cfu/mL of V. harveyi by immersion method for three, six, 12, 24, 48 and 72 hours. The presence of hemolisin genes was detected by PCR techniques. The electrophoresis detected narrow hemolysin genes after PL were exposed for three and six hours. Clear visible bands of DNA Vibrio were observed for 12 hours of exposure. In contrast, no detected hemolysin gene of Vibrio was observed for PL exposed within 24, 48, and 72 hours. The rapid detection on Vibrio pathogenic for tiger shrimp PL should be conducted within three to 12 hours of exposure. No recommendation in utilizing this rapid detection for tiger shrimp PL exposed beyond 12 hours of V. harveyi.

Key concepts: Penaeus monodon, Vibrio harveyi, Shrimp, Biology, Microbiology, Hemolysin, Vibrio parahaemolyticus, Vibrio

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Deteksi Vibrio harveyi menggunakan primer hemolisin pada benur udang windu Penaeus monodon Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon — Research Paper | ScholarLens