Aerobic bacteriological profile with antibiogram of pus isolates

Background: Pyogenic wound infection is major cause of morbidity. Difficulty in treatment of such infection is because of emergence of multidrug resistant strains. To avoid such situation, it is very important for right and empirical choice of antibiotics. Hence it is mandatory for every pus samples to undergo culture and sensitivity. Objectives: 1.        To identify and isolate aerobic bacteria from pus samples. 2.        To study antibiotic profile of aerobic bacteria. Methods: A cross sectional study was conducted in Department of Microbiology of a private tertiary care hospital in Vijaypur. Total of 256 pus samples send for aerobic culture and sensitivity from different departments to Department of Microbiology from January 2015 to December 2015 were included in the study. Aerobic bacteria isolated, identified and antibiotic profile was determined from pus samples using standard protocol. Data was analyzed using MS Excel 2010. Results and interpretation: In our study among 256 pus samples send from various departments, 169(66.01%) showed positive aerobic growth. Gram negative bacteria outnumbered the gram positive isolates. Commonest isolate was Staphylococcus aureus followed by Pseudomonas.  30(39.47%) of MRSA were isolated. All Staphylococcus aureus were sensitive to Vancomycin and linezolid. Among 48 Pseudomonas isolated Polymyxin B and Piperacillin-Tazobactam were effective drugs. In enterobacteriaceae group most effective antibiotic was Meropenam. Conclusion: It is important for a clinician to send all the pus sample for microbiological analysis and their antibiogram before putting cases on antibiotics so that emergence of drug resistance can be minimized. Key words: Pus, Antibiogram, Aerobic bacteria, MRSA, Pseudomonas