Hyperthermia Combined with Dipyridamole Enhance the Cytotoxicity of 5-Fluorouracil both in Vitro and in Vivo.

The combined effect of 5-FU, DP, and HT was investigated both in vitro and in vivo. When HeLa and B16 melanoma cells were exposed concomitantly ti heat (43' C, 40min) and DP (2.5 μg/ml), the cytotoxicity of 5-FU (0.1 μg/ml) was sugnificantly enhanced determined with colonergic assay. SDI test also revealed that this trimodality treatment was more cytotoxic to B16 melanoma cells and human cancer tissues than 5-FU alone.Growth of B16 melanoma that had been implanted subcutaneously was inhibited by DP (100mg/kg), and 5-FU (13.2mg/kg), when compared with 5-FU alone.Hyperthermia (HT) and dipyridamole (DP) augment the cytotoxicity of 5-fluorouracil (5-FU) in vitro and in vivo. The combination therapy of 5-FU, DP and HT is thought to be useful toward malignant diseases.