Review and Optimization of Linearity and Precision in Quantitative HPLC-ELSD with Chemometrics

Recent improvements in ELSD instrumentation and a better understanding of the limitations and advantages of this detection method are increasing its popularity, especially for the analysis of molecules lacking chromophores and as an economical surrogate mass spectrometry detector during method development. However, ELSD instrument settings must be appropriate for the physicochemical properties of the analyte and analyte-specific standard. Also, the nonlinear response of this detection method in gradient elution requires careful calibration and proper chemometrics to obtain reliable quantitation.