2016•Annals of Clinical and Translational NeurologyOpen access
Alpha‐synuclein RT ‐Qu IC in the CSF of patients with alpha‐synucleinopathies
Graham Fairfoul, Lynne I. McGuire, Suvankar Pal, James W. Ironside, Juliane Neumann, Sharon Christie, Catherine Joachim, Margaret M. Esiri, Samuel Evetts, Michal Rolinski, Fahd Baig, Claudio Ruffmann, Richard Wade‐Martins, Michele T. M. Hu, Laura Parkkinen, Alison J. E. Green
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Abstract
We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.
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