The Effects of Biopesticide Azadirachtin on the Fifth Instar Galleria mellonella L. (Lepidoptera: Pyralidae) Larval Integument

The chitin synthesis inhibitory (CSI) effects of azadirachtin on the fifth instar Galleria mellonella L. larval integument were investigated. Using probit analysis, the LC95 value for G. mellonella larvae was detected as 3991 ppm. In this study, 3991 ppm and the reduced 1995, 997, and 498 ppm concentrations were used. It was observed that azadirachtin had different effects on the integument when the larvae were fed semi-synthetic feed containing 498, 997, 1995, or 3991 ppm azadirachtin. Morphologically failed molting, disruption of cuticle structure, hemolymph loss, and darkening were observed in azadirachtin-administered larvae. In the experimental groups, depending on the dose/time relationship, high doses of azadirachtin blocked cuticle secretion in higher levels. In larvae fed a diet containing azadirachtin, cuticle secretion was reduced by 21%–24% compared with the control group.