Immunoaffinity Chromatography as a Tool for the Analysis of Antibiotics and Sulfonamides

Monoclonal antibodies against streptomycin, sulfamethazine, and sulfadiazine were used for the preparation of immunoaffinity chromatography (IAC) columns. The capacity of the resulting columns was approximately 250, 1050, and 1050 ng per column for sulfadiazine, sulfamethazine (sulfadimidine), and streptomycin, respectively. Artificially contaminated milk samples were used for the performance testing of the columns under practical conditions. Between 84.2 and 96.7% of sulfadiazine, sulfathiazole, and sulfamerazine were bound by the column containing the monoclonal antibody against sulfadiazine. 89.8% of the applied sulfamethazine was retained by the respective column. The streptomycin column bound 80.4% and 88.7% of milk samples containing 100 ppb streptomycin and dihydrostreptomycin, respectively. A multi-immunoaffinity chromatography (MIAC) column for sulfonamides bound between 78.6 and 98.7% of a mixture of sulfadiazine, sulfathiazole, sulfamerazine, and sulfamethazine (100 ng each).