Effects of nitrogenous fertilizer strategies on sugar components in the stubbles of Zea mexicana.

High performance liquid chromatography(HPLC) technology was used to measure sugar content in the stubbles of Zea mexicana.The effect of nitrogen on sugar content and the relationship between sugar content and regeneration in the stubbles was analyzed and showed that the soluble sugar content of Z.mexicana.Following one nitrogen application was significantly higher than that of the CK,which was higher than that of the three nitrogen applications treatment.The sugar content decreased as the amount of nitrogen increased.There were five soluble sugars(WSP,sucrose,glucose,fructose and mannitol) in the stubbles of Z.mexicana,and after nitrogen application,the contents of WSP,sucrose,glucose and fructose was lower than that of the CK.The sucrose and glucose content decreased with an increase of nitrogen but the fructose content increased.Regression analysis showed that sucrose,fructose and mannitol were the principal factors that affected the regeneration of Z.mexicana.The sucrose and mannitol content decreased while fructose rose during the H1 period but sucrose content rose while fructose and mannitol contents decreased during the H2 period and were favorable for regrowth.The CK dry matter yield of the regrowth was the lowest of the treatments and the highest biomass was obtained using a low nitrogen quantity(300 kg/hm2) applied once or a high nitrogen quantity(600 kg/hm2) applied three times.