Electrokinetic Particle Translocation through a Nanopore Containing a Floating Electrode

The effect of the particle-nanopore electrostatic interaction highly depends on the ratio of the particle size to the electrical double layer (EDL) thickness. When the EDLs of the DNA nanoparticle and the nanopore wall are overlapped and the electric field imposed is relatively low, the effect of the particle-nanopore electrostatic interaction dominates over the EOF effect, which might lead to DNA nanoparticle trapping inside the nanopore. However, the particle-nanopore electrostatic interaction is negligible if the EDLs are not overlapped. The nanofluidic FET offers a flexible and electrically compatible approach to control DNA nanoparticle translocation actively through a nanopore for DNA sequencing.