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Gel Permeation Chromatography

Augustus C. Ouano, Edward M. Barrall, A. Broido, A. C. Javier‐Son

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Abstract

Cellulose samples which have undergone various stages of thermal decomposition were characterized for changes in molecular weight and molecular weight distribution using gel permeation chromatography (GPC) and viscometry. Calculation of cellulose molecular weights (as cellulose nitrate) from the chromatogram and polystyrene calibration curves using the extended chain length-retention volume relationship (Q factor) resulted in very poor agreement between GPC and viscometric molecular weight values. Molecular weight averages determined by GPC were approximately five times greater than those obtained by viscometric technique. Application of various hydrodynamic considerations completely corrected this problem. The effects of calibration standard distribution and range are also discussed.

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What this paper is about

Cellulose samples which have undergone various stages of thermal decomposition were characterized for changes in molecular weight and molecular weight distribution using gel permeation chromatography (GPC) and viscometry. Calculation of cellulose molecular weights (as cellulose nitrate) from the chromatogram and polystyrene calibration curves using the extended chain length-retention volume relationship (Q factor) resulted in very poor agreement between GPC and viscometric molecular weight values. Molecular weight averages determined by GPC were approximately five times greater than those obtained by viscometric technique. Application of various hydrodynamic considerations completely corrected this problem. The effects of calibration standard distribution and range are also discussed.

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Available abstract

Cellulose samples which have undergone various stages of thermal decomposition were characterized for changes in molecular weight and molecular weight distribution using gel permeation chromatography (GPC) and viscometry. Calculation of cellulose molecular weights (as cellulose nitrate) from the chromatogram and polystyrene calibration curves using the extended chain length-retention volume relationship (Q factor) resulted in very poor agreement between GPC and viscometric molecular weight values. Molecular weight averages determined by GPC were approximately five times greater than those obtained by viscometric technique. Application of various hydrodynamic considerations completely corrected this problem. The effects of calibration standard distribution and range are also discussed.

Key concepts: Gel permeation chromatography, Molar mass distribution, Polystyrene, Cellulose, Chromatography, Viscometer, Chemistry, Calibration curve

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