Enzyme assisted extraction of polysaccharides from Flammulina velutipes and its radicals scavenging activity

ABSTRACT: An efficient enzyme-assisted extraction procedure was developed and optimized for the extraction of polysaccharides from Flammulina Velutipes (FVPs). Based on the single-factor experiments, Response Surface Methodology (RSM) was used to estimate and optimize the experimental variables. The results showed that the optimal extraction conditions for the polysaccharides were extraction temperature 55.0 °C, extraction pH 5.24, and extraction time 3.4 h. Under these conditions, the experimental yield of polysaccharides was 8.60 ± 0.22%, which was close with the predicted yield value (8.66%). In addition, the radicals scavenging activity of the FVPs were investigated in vitro. The results showed that FVPs possessed significant inhibitory effects on 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical. These results suggested that enzyme-assisted extraction technology is a great extraction technology on the polysaccharides from F. velutipes, and FVPs could be a suitable natural antioxidant and may be the functional foods for humans.