1991•American Journal of Enology and ViticultureRequires access

Fractionation of Phenolic Compounds From Grapes by Size Exclusion Liquid Chromatography With HPLC Instrumentation

Juan Cacho, J. Enrique Castells

Abstract

Several commercial HPLC columns and gels were studied in order to fractionate phenolic compounds from grapes by molecular size. Sephadex G type were the most appropriate for the fractionation and it could be used with HPLC instrumentation to allow rapid, reproducible separations in which all the process variables were perfectly controlled. Sephadex G-10, G-15, and G-25 were applied to study the evolution of condensed phenolic compounds during the maturation of Garnacha grapes in 1987 and 1988.

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Key concepts: Chromatography, Fractionation, High-performance liquid chromatography, Chemistry, Sephadex, Size-exclusion chromatography, Organic chemistry, Enzyme