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An Enzymatic Assay for Sorbitol in Apple Organs

Christopher S. Brown, Eric Young, David M. Pharr

Abstract

Abstract An enzymatic assay for sorbitol in organs of apple ( Malus domestica Borkh.) is described. Sorbitol concentrations in ethanolic extracts of apple leaves, stems, roots, and fruit can be measured by detecting optical density changes at 340 nm resulting from the reduction of NAD + coupled with the conversion of sorbitol to D-fructose by sorbitol dehydrogenase. The assay is unaffected by normal concentrations of fructose, glucose, or sucrose in the tissue. Sorbitol concentrations determined by the enzymatic assay were verified by HPLC analysis of the same tissue samples.

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Key concepts: Sorbitol, Sorbitol dehydrogenase, Malus, Fructose, Sucrose, Chemistry, Enzyme, Biochemistry