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In vitro shoot multiplication from nodal explants of Boucerosia diffusa Wight—An endemic medicinal plant

T Ramadevi, A. Ugraiah, T. Pullaiah

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Abstract

In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 μM) and repeated subculture on MS+BAP (8.87 μM)+TDZ (2.27 μM) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 μM). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help ex situ conservation of this endemic species.

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What this paper is about

In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 μM) and repeated subculture on MS+BAP (8.87 μM)+TDZ (2.27 μM) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 μM). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help ex situ conservation of this endemic species.

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Available abstract

In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 μM) and repeated subculture on MS+BAP (8.87 μM)+TDZ (2.27 μM) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 μM). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help ex situ conservation of this endemic species.

Key concepts: Kinetin, Explant culture, Shoot, Micropropagation, Subculture (biology), Apocynaceae, Biology, Thidiazuron

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