1995•ACS symposium seriesRequires access
Separation of Three Alkaloids from Senecio fuberi Hemsl by High-Speed Countercurrent Chromatography
D.-G. Cai, Meijuan Gu, Guanghui Zhu, J.-D. Zhang, Tao Jin, Tong Zhang, Yoichiro Ito
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Abstract
Three alkaloids, squalidine, platyphylline, and neoplatyphylline from Senecio fuberi Hemsl, were completely separated by high-speed counter-current chromatography (CCC). Separation was performed with a two-phase solvent system composed of chloroform/0.07M sodium phosphate-0.04M citrate buffer (pH 6.20-6.45) (1:1) in which the upper aqueous phase served as the stationary phase, and the lower chloroform phase as the mobile phase. Each alkaloid was identified by the physical and chemical constants as well as TLC and mass spectral data. Platyphylline and neoplatyphylline are cis-trans isomers which are difficult to separate by other means on a preparative scale.