Formation and cell wall regeneration of protoplasts from Schizophyllum commune

Osmotically sensitive protoplasts were released from the mycelium of the basidiomycete Schizophyllum commune through the action ofan extracellular enzyme preparation isolated from the culture filtrate of Trichoderma viride (recently renamed T. harzianum) grown on hyphal walls of the former organism. The conditions for obtaining stable protoplasts were determined. Maximum numbers of protoplasts were released from young growing mycelium by using 0.5 M MgSO4 or 0.3 M KCI in the presence of 0.05 M Na-maleate buffer at pH 5.8 (osmotic potential - 15.8 atm). Protoplasts emerged through ruptures in the wall, initially at the apices, but later also from older parts of the hyphae... Zie: Summary