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Purification of DNA Topoisomerases and Inhibition by Fluoroquinolones

John T. George, Ian Morrissey

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Abstract

Fluoroquinolones act by inhibiting the essential type II topoisomerases, DNA gyrase and topoisomerase IV ( 1 ). DNA gyrase is a tetramer composed of 2 A subunits and 2 B-subunits encoded for by gyrA and gyrB , respectively. This enzyme is the only enzyme known that is capable of negatively supercoiling DNA ( 2 ), a process essential for the bacterium to contain its DNA within the confines of the cell. Topoisomerase IV is also a tetramer composed of 2 C subunits and 2-E subunits encoded for by the parC and parE genes. This enzyme is involved in the unlinking of daughter chromosomes following replication ( 3 ). These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

About this research paper

What this paper is about

Fluoroquinolones act by inhibiting the essential type II topoisomerases, DNA gyrase and topoisomerase IV ( 1 ). DNA gyrase is a tetramer composed of 2 A subunits and 2 B-subunits encoded for by gyrA and gyrB , respectively. This enzyme is the only enzyme known that is capable of negatively supercoiling DNA ( 2 ), a process essential for the bacterium to contain its DNA within the confines of the cell. Topoisomerase IV is also a tetramer composed of 2 C subunits and 2-E subunits encoded for by the parC and parE genes. This enzyme is involved in the unlinking of daughter chromosomes following replication ( 3 ). These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Available abstract

Fluoroquinolones act by inhibiting the essential type II topoisomerases, DNA gyrase and topoisomerase IV ( 1 ). DNA gyrase is a tetramer composed of 2 A subunits and 2 B-subunits encoded for by gyrA and gyrB , respectively. This enzyme is the only enzyme known that is capable of negatively supercoiling DNA ( 2 ), a process essential for the bacterium to contain its DNA within the confines of the cell. Topoisomerase IV is also a tetramer composed of 2 C subunits and 2-E subunits encoded for by the parC and parE genes. This enzyme is involved in the unlinking of daughter chromosomes following replication ( 3 ). These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Key concepts: DNA gyrase, Topoisomerase, DNA supercoil, Tetramer, Topoisomerase IV, Circular bacterial chromosome, DNA, DNA replication

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